Isolation And Characterization Of Tinea Captis From Hair Barbing Equipment

In Enugu Metropolis

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ABSTRACT
This study was done on the isolation and characterization of Tinea Capitis, from hair barbing equipment in Enugu metropolis.
A random sampling was made of 150 hair barbing equipment consisting of hair brushes, hair clippes, combs, aprons and theatre seats.
There were categorized according to location and types of hair barbing salon. 60 sample were positive for dermatophytes and these included Microporum audouim 24 (40%), Trichophyton rubrum 16 (26.7%), I. Mentargrophyter 10 (16.7% and I. Soudanose 10 (16.7%).
Of the 75, modern salon samples 16 were positive (21.3%)L The Ogui/Obiagu zone had the highest positive samples for Tinea Capitis with 19 out of 30 positive samples (31.7%0. This was followed by Agbani Road/Uwani which recorded 14 out of 30 positive (23.3%) Emene/Abakpa 11 out of 30 (18.3%), New Heaven Independence layout 9 out of 30 (15%) and GRA/Trans Ekulu 7 our of 30 (11.7%). It therefore appeared that percentage incidence increased with decreased in standard of living.

TABLE OF CONTENTS

CHAPTER ONE:
Introduction 1
1.1 Background Information 1
1.2 Aim and Objective of the Study 5
1.3 Statement of Problems 5
1.4 Justification of the Study 6
1.5 Limitation of the Study 7

CHAPTER TWO:
Literature Review 8
2.1 Clinical Manifestation of Tinea Capitis 8
2.2 Age and Sex Incidence 9
2.3 General Incidence of Tinea Capitis
with Particular Reference to Africa 10
2.4 Differential Diagnoses 12
2.5 Therapy of Tinea Capitis 13

CHAPTER THREE
3.0 Methodology 17
3.1 Study Area 17
3.2 Types of Hair Barbing Salon and Locality 18
3.3 Material and Regents for the Study 19
3.3.1 List of Materials 19
3.3.2 List of Reagents 19
3.4 Data Collection 19
3.4.1 On Subject 20
3.5 Analytical Techniques used for the Study 20
3.5.1 Methods of Identification 20
3.5.2 Biochemistry Test used for the Study 22
3.6 Characteristics of Dermatophytes Identified 23

CHAPTER FOUR:
Result and Discussions 26
4.1 Result 26
4.2 Discussions 29

CHAPTER FIVE:
Conclusion and Recommendations 32
5.1 Conclusion 32
5.2 Recommendation 32
References 34
Appendix 36

CHAPTER ONE

INTRODUCTION
1.1 BACKGROUND INFORMATION
Barbing which is the act of removing the hair from the head has diverse economic liabilities. This includes dissemination of diseases and beauty. In the case of disease, the microorganisms of Tinea Capitis is usually found in the hair. This organism belongs to the fungi groups.

Fungi constitute a group of non-motile eukaryotic organisms that have a definite cell wall, devoid of chlorophyll and reproduce by means of spores. The spores can be produced sexually or asexually and it is propagating granule with or without embryo. Fugal spores germinate on appropriate substrate to produce morphologically such diverse forms as mould and yeast.

In moulds, spores germinate to produce branching filaments called hyhae, which may be divided, into cell by septa. In yeast on the other hand, the spores germinate to produce round, oval embryonated single cell that reproduced mostly by budding and form moist or muciod colonies. (Kwong Chung and Benette, 1992).

According to Jawetz et al (1989), as the hyphae continues to grow and branch a mat of growth that projects above the surface of the substrate is called the vegetative mycelium. Most fungi reproduce by forming spore through mitosis fungi with only asexual spores formation are called fungi imperfecti.

Fungi have diverse groups among which area specialized group known as dermatophytes. These dermatophytes infect keratinized tissue that is the epidermis of skin, hair and nail causing the disease dermatophytosis. The dermatophytes produce infection with mild to severe symptoms depending on the immunological response of the host. In general, they do not invade subcutaneous or deep tissues. Granulnatous lesions in subcutaneous cause both with and without the granule formation that is characteristic of mycetomas have been occasionally reported.

Dermatophytes are also known as ‘ringworm’, fungi. The name ringworm has been in use at least from the sixteenth century and it was carried to describe the circular lesion produced by dermatophyte on the skin or scalp (Kwong Chung and Benette, 1992). Ringworm is clinically referred to as Tinea and locations involved are usually the surface of the body (Tinea Copons), of the groin (Tinea Gruris) of the bearded area (Tinea Barbae), of the scalp (Tinea capitis) (Cheesbrough, 1984).

Tinea Capitis, ringworm of the scalp and hair may be caused by any of the Dermatophytes pathogenic for man except Epidermophyton Flocossum and Trichophyton Concentricum. The disease affects mainly children although some species of fungus may attack adult scalp or continue from childhood into adult life.

The most chronic and persistent form of Tinea Captis are caused by several anthropophilic species. Chronity seems to be related to the relative absence of inflammatory response. Anthropohilic ringworm in children is transmitted by contact and may assume epidemic proportion. Hair gears fomites, barbe’s instrument and even theatre seats have been shown to be contaminated with the fugus. Eperdermis occur mainly in school boarding schools, campus and in center of over crowding and poor hygiene.

The spores of microsporum audouinii and other dermatophtes have been recovered from the air of school rooms and other suspected localities. In a work carried out hair brushes by (Mackenzie et al, 1960) in Belfast, it was shown that clinical examination alone was inadequate for the detection of versions that are trival or virtually absent. Individuals with only discrete hairs infected can be regard for practical purpose as carriers, although symptom less, they disseminate infection over a period of years and can be responsible for a gradual build up of infection potential. To the best of my knowledge, no work has been documented on the isolation and characterized of Tinea Capitis from hair barbing equipment in Enugu metropolis. It has been observed that with the introduction of modern hair barbing equipment consisting of modern barbing kits, there has been increased incidence of kerion, like lessons. In the less severe infection, the individual infected follicles may be discerned. (Kwong Chung And Benette, 1992). These are realized particularly on the part of the scalp just above the neck region. This…

Isolation And Characterization Of Tinea Captis From Hair Barbing Equipment

In Enugu Metropolis

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