THE EFFECT OF CISSAMPELOS MUCRONATA ON Na+/K+ ATPase IN THE KIDNEY AND SERUM OF WISTAR RAT

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CHAPTER ONE

1.0   INTRODUCTION

1.1   BACKGROUND OF THE STUDY

Cissampelosmucronata belongs to the family Menispermaceaeand is highly specialized in its extraordinary rich diversification of benzyltetrahydroisoquinoline and aporphine derivatives (Watson and Dallwitz, 1992). Because of its richness in alkaloids, this family is used worldwide in traditional medicine to treat a variety of ailments. It is used to facilitate childbirth, as an abortifacient, as an emmenagogue, to treat stomach pains and as a diuretic (Burkill,et al 1997). It’s fresh aerial parts are used to regulate menstruation (Elujoba,et al 1995). Ash obtained from the leaves, twigs and root bark are eaten to treat side pain (Baerts and Lehmann, 1989) and the sap of the whole plant is mixed with HeliotropiumIndicum L. and drunk to treat leucorrhea (Adjanohoun, et al 1989).

Decoction of the stem bark and leaves is also drunk to calm nerves (Nwosu, et al 1999). The root is used to treat abdominal pains, swollen stomach and gastro-intestinal upset due to bewitchment (Gelfand et al 1985; Chhabra et al 1990). It is also used to prevent or arrest uterine hemorrhage, painful uterus, to treat infertility, to prevent abortion (Gelfand et al 1985; Vanwyk and Gericke, 2000) and to treat conjunctivitis (Tshibangu, et al 2002).

In Nigeria, like many African countries Cissampelosmucronata is usually prepared in the form of infusions, decoctions, tinctures or syrups in the treatment of various ailments.

Its indigenous names in Nigeria include;

TRIBE	NAMES
Hausa	Jibdar kasa or Damaji
Igbo	Abakenwo
Babur/Bura	Kwahara or Kwahirka
English	Ivy vine

 

Despite its various uses during pregnancy a search conducted for embryo fetal (developmental) effect of Cissampelosmucronata found no results. Against this background the study was designed to examine the effect of Cissampelosmucronata on Na K ATPase on the kidney and the serum of wistar rats.

The kidney and the serum are structurally complex organs that has evolved to sub serve a number of important functions; excretion of waste products of metabolism, regulation of body water and salt, maintenance of appropriate acid balance and secretion of a variety of hormones and autacoids in man (Kumar et al, 2000).

1.2   AIMS AND OBJECTIVES OF THE STUDY

AIMS

The aims and objectives of this study are to;

• To determine the effect of Cissampelosmucronata on Na+/K+ ATPase activity in the serum and kidney of wistar rats.

OBJECTIVES

• To assay for Na+/K+ ATPase
• Estimation of Phosphorus

 

 

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